Structure of the 70S ribosome complexed with mRNA and tRNA.
نویسندگان
چکیده
The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.
منابع مشابه
How do the ends replicate?
20 Schmeing, T.M. et al. (2005) An induced-fit mechanism to promote peptide bond formation and exclude hydrolysis of peptidyl-tRNA. Nature 438, 520–524 21 Weixlbaumer, A. et al. (2008) Insights into translational termination from the structure of RF2 bound to the ribosome. Science 322, 953–956 22 Laurberg, M. et al. (2008) Structural basis for translation termination on the 70S ribosome. Nature...
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ورودعنوان ژورنال:
- Science
دوره 313 5795 شماره
صفحات -
تاریخ انتشار 2006